Search Results (36)

(1) Background: Given the widespread use of Alkalihalobacillus clausii (A. clausii) as a probiotic in recent decades and the detection of bacteremia cases in a group of patients, we sought to analyze cases of A. clausii bacteremia following oral probiotic use (2) Methods: A retrospective observational study was conducted at a private hospital in San Jose, Costa Rica. Cases of bacteremia caused by A. clausii confirmed by the microbiology laboratory were analyzed in patients who received oral treatment with this probiotic between January 2020 and January 2022. In addition, an isolate (HCB-AC2) was compared through whole genome sequencing to demonstrate the correlation of bacteremia and A. clausii. Possible vulnerability factors related to the development of this condition were determined. (3) Results: Four cases were identified in this hospital over 2 years. Genomic analysis of isolate HCB-AC2, using two different methods, showed identical results. This indicates that HCB-AC2 is genomically identical to ENTpro and the Enterogermina^® reference genome. The median age was 71 years, and all patients had some degree of immunosuppression. All patients met at least three sepsis criteria at the time of bacterial identification. Most patients were treated with vancomycin and levofloxacin. Three of the identified patients died. (4) Conclusion: A. clausii can be used as a probiotic, but caution is advised when used in immunosuppressed and elderly patients. These findings align with those reported in similar case studies. Full article

The presence of nontuberculous mycobacteria in biofilms on the surface of medical devices may affect the opportunistic pathogens that are common inhabitants of such biofilms. This study assessed the effect of Mycolicibacterium iranicum cell-free supernatants on biofilm formation and antibiotic susceptibility of Escherichia coli and Staphylococcus epidermidis differing in the anti-hemoglobin activity level. The cell-free supernatants have been shown to stimulate biofilm formation and also help reduce susceptibility of opportunistic pathogens to a number of antibiotics. Full article

Klebsiella pneumoniae, a Gram-negative pathogen, poses a significant threat as a cause of community- and hospital-acquired infections worldwide. The emergence of multidrug-resistant strains, particularly in nosocomial settings, has further complicated the management of these infections. This study aimed to investigate the culture identification and antibiogram of K. pneumoniae isolated from sputum samples collected in various districts in Mardan, Khyber Pakhtunkhwa, Pakistan. A total of 16 sputum samples were collected from patients at the Mardan Medical Complex. Standard microbiological techniques were employed to identify K. pneumoniae, and the antibiotic susceptibility testing was performed using the Kirby–Bauer disc diffusion method, following CLSI guidelines. Among the confirmed K. pneumoniae isolates, approximately 50% were found to be multidrug-resistant. The results indicated resistance to several antibiotics, including vancomycin (30 g), amikacin (30 g), chloramphenicol (30 g), amoxicillin (30 g), and ticarcillin (75 g), while being susceptible to meropenem (10 g), piperacillin (100 g), and tazobactam (110 g). A bioinformatics analysis was also conducted to gain deeper insights into the resistance patterns and potential clustering of isolates. This comprehensive study provides valuable information on the epidemiological trends and antimicrobial susceptibility profile of K. pneumoniae in the region. The findings of this study highlight the urgent need for antimicrobial stewardship programs to combat the rising challenge of antibiotic resistance. Understanding the resistance landscape of K. pneumoniae can guide healthcare professionals in selecting appropriate antibiotics and improving patient outcomes. These data can contribute to the formulation of local antibiotic policies and assist clinicians in making rational choices for antibiotic therapy. Full article

During meat processing, lactic acid bacteria (LAB) have to competitively adapt to the hostile environment produced by curing additives (CA). The objective of this study was to investigate the ability of Latilactobacillus curvatus CRL 705, a bioprotective strain of meat origin, to adapt to CA. A physiological and proteomic approach was performed. CRL 705 was grown in a chemically defined medium (CDM) containing specific concentrations of CA (NaCl, nitrite, sucrose, and ascorbic acid). The results showed minor differences in growth kinetics in the presence of CA. Glucose consumption, present in the CDM, and production of lactic acid and bacteriocins were not significantly affected. Proteomic analyses indicated that most of the identified proteins (36 out of 39) mainly related to carbohydrate metabolism (18%), posttranslational modifications (15.6%), energy production and conversion (11.1%), translation (11.1%), and nucleotide metabolism (8.9%) were underexpressed. In response to the studied CA, CRL 705 slowed down its general metabolism, achieving slight changes in physiological and proteomic parameters. The observed performance is another characteristic that extends the well-known competitive profile of CRL 705 as a meat starter and bioprotective culture. This is the first report dealing with the impact of CA on LAB proteomics. Full article

Manganese (Mn) is essential for plant growth, as it serves as a cofactor for enzymes involved in photosynthesis, antioxidant synthesis, and defense against pathogens. It also plays a role in nutrient uptake, root growth, and soil microbial communities. However, the availability of Mn in the soil can be limited due to factors like soil pH, redox potential, organic matter content, and mineralogy. The excessive use of chemical fertilizers containing Mn can lead to negative consequences for soil and environmental health, such as soil and water pollution. Recent research highlights the significance of microbial interactions in enhancing Mn uptake in plants, offering a more environmentally friendly approach to address Mn deficiencies. Microbes employ various strategies, including pH reduction, organic acid production, and the promotion of root growth, to increase Mn bioavailability. They also produce siderophores, anti-pathogenic compounds, and form symbiotic relationships with plants, thereby facilitating Mn uptake, transport, and stimulating plant growth, while minimizing negative environmental impacts. This review explores the factors impacting the mobility of Mn in soil and plants, and highlights the problems caused by the scarcity of Mn in the soil and the use of chemical fertilizers, including the consequences. Furthermore, it investigates the potential of different soil microbes in addressing these challenges using environmentally friendly methods. This review suggests that microbial interactions could be a promising strategy for improving Mn uptake in plants, resulting in enhanced agricultural productivity and environmental sustainability. However, further research is needed to fully understand these interactions’ mechanisms and optimize their use in agricultural practices. Full article

Microorganisms have the potential to address environmental pollution, but the interaction mechanism between microorganisms and mine tailings is not well understood. This work was aimed at determining the bacterial isolates in soils and mine tailings and evaluating the distribution of metals, antimony (Sb), and arsenic (As) in the soils around the Komsomolsk tailings. Areas with high concentrations of As, Sb, cadmium (Cd), and lead (Pb) were found. Assessment based on the value of the contamination factor (CF) indicated large-scale As, Sb, Pb, Cd, iron (Fe), bismuth (Bi), and beryllium (Be) pollution, especially in soils sampled from the northeast direction of the mine tailings. Soils had a higher number of CFUs per g of dry weight than did the tailings, ranging from 84 × 10⁶ to 3.1 × 10⁹ and from 20 × 10⁶ to 1.7 × 10⁹, respectively. Arsenic exhibited a positive statistical correlation with the number of CFUs of Agrococcus and Staphylococcus. In addition, a positive correlation was found between the concentration of Co and the number of CFUs of Moraxella and Microbacterium. The Sb exhibited a positive correlation with Streptomyces. These results can be used to develop methods for waste reclamation, including the use of isolated bacterial strains for arsenic removal by precipitation. Full article

Phosphorus (P) is one of the most important elements required for crop production. The ideal soil pH for its absorption by plants is about 6.5, but in alkaline and acidic soils, most of the consumed P forms an insoluble complex with calcium, iron, and aluminum elements and its availability for absorption by the plant decreases. The supply of P needed by plants is mainly achieved through chemical fertilizers; however, in addition to the high price of these fertilizers, in the long run, their destructive effects will affect the soil and the environment. The use of cheap and abundant resources such as rock phosphate (RP) can be an alternative strategy for P chemical fertilizers, but the solubilization of P of this source has been a challenge for agricultural researchers. For this, physical and chemical treatments have been used, but the solution that has recently attracted the attention of the researchers is to use the potential of rhizobacteria to solubilize RP and supply P to plants by this method. These microorganisms, via. mechanisms such as proton secretion, organic and mineral acid production, siderophore production, etc., lead to the solubilization of RP, and by releasing its P, they improve the quantitative and qualitative performance of agricultural products. In this review, addressing the potential of rhizosphere microbes (with a focus on rhizobacteria) as an eco-friendly strategy for RP solubilization, along with physical and chemical solutions, has been attempted. Full article

Extremophilic microorganisms such as those that thrive in high-salt and high-alkaline environments are promising candidates for the recovery of useful biomaterials including polyhydroxyalkanoates (PHAs). PHAs are ideal alternatives to synthetic plastics because they are biodegradable, biocompatible, and environmentally friendly. This work was aimed at conducting a bioprospection of bacteria isolated from hypersaline-alkaliphilic lakes in Kenya for the potential production of PHAs. In the present study, 218 isolates were screened by Sudan Black B and Nile Red A staining. Of these isolates, 31 were positive for PHA production and were characterized using morphological, biochemical, and molecular methods. Through 16S rRNA sequencing, we found that the isolates belonged to the genera Arthrobacter spp., Bacillus spp., Exiguobacterium spp., Halomonas spp., Paracoccus spp., and Rhodobaca spp. Preliminary experiments revealed that Bacillus sp. JSM-1684023 isolated from Lake Magadi had the highest PHA accumulation ability, with an initial biomass-to-PHA conversion rate of 19.14% on a 2% glucose substrate. Under optimized fermentation conditions, MO22 had a maximum PHA concentration of 0.516 g/L from 1.99 g/L of cell dry weight and 25.9% PHA conversion, equivalent to a PHA yield of 0.02 g/g of biomass. The optimal PHA production media had an initial pH of 9.0, temperature of 35 °C, salinity of 3%, and an incubation period of 48 h with 2.5% sucrose and 0.1% peptone as carbon and nitrogen sources, respectively. This study suggests that bacteria isolated from hypersaline and alkaliphilic tropical lakes are promising candidates for the production of polyhydroxyalkanoates. Full article

The establishment of Permanent Preservation Plots (PPPs) in natural forests has a signifi-cant role in assessing the impact of climate change on forests. To pursue long-term studies on cli-mate change, PPPs were established during the year 2016 in two major forest areas in Bangalore to conduct ecological studies to monitor the vegetation changes. One of the objectives of the study was to understand the drivers of diversity, such as soils, in terms of nutrients and physical and biological properties. The native tropical forest of Bangalore, which houses Bannerghatta National Park (BNP) on the outskirts, is relatively underexplored in terms of its microflora, particularly arbuscular my-corrhizal fungi (AMF). Hence, the present study was aimed at the quantitative estimation of arbus-cular mycorrhizal fungi (AMF) in the three 1-ha PPPs which were established in Bannerughatta National Park (BNP) and Doresanipalya Reserve Forest (DRF) as per the Centre for Tropical Forest Sciences (CTFS) protocol. In BNP, two plots were established, one in the Thalewood house area (mixed, moist, deciduous type) and the other in the Bugurikallu area (dry, deciduous type). In DRF, one plot was established in dry, deciduous vegetation. Each one-hectare plot (100 m × 100 m) was subdivided into twenty-five sub-plots (20 m × 20 m). Composite soil samples were collected during two seasons (dry and wet) and analyzed for AMF spore and available phosphorus (P) content. The results revealed the presence of AMF in all the three plots. Doresanipalya plo had the highest spore number, followed by the Bugurikallu plot and Thalewood house plot. The available phosphorous and AMF spore numbers showed correlations in all the three plots. Among the AMF spores, the Glomus species was found to dominate in all the three plots. The study shows that the dry, decidu-ous forests accommodated more AMF spores than the mixed, moist forests. Full article

Antimicrobial resistance (AMR) is a global public health threat. Quality data on AMR are needed to tackle the rise of multidrug-resistant clones. These data are rare in low-income countries, especially in sub-Saharan Africa. In this study, we investigated the rise of extended-spectrum β-lactamase–producing (ESBL) Enterobacteriaceae in Bangui, Central African Republic. We collected 278 fecal samples from 0–5-year-old children admitted to the Pediatric University Hospital Complex in Bangui from July to September 2021. Enterobacteriaceae were isolated and identified, and their susceptibility to 19 antibiotics was tested. We recovered one and two Enterobacteriaceae species from 208 and 29 samples, respectively. One clone of each species from each sample was further characterized, for a total of 266 isolates. Escherichia coli predominated, followed by Klebsiella. AMR was frequent, with 98.5% (262/266) of the isolates resistant to at least one antibiotic. Additionally, 89.5% (238/266) of the isolates were multidrug resistant, with resistance being frequent against all tested antibiotics except carbapenems and tigecycline, for which no resistance was found. Importantly, 71.2% (198/278) of the children carried at least one ESBL species, and 85.3% (227/266) of the isolates displayed this phenotype. This study confirms the rise of ESBL Enterobacteriaceae in Bangui and stresses the need for action to preserve the efficacy of antibiotics, as crucial for the treatment of bacterial infections. Full article

Traditional preparation of African indigenous vegetables (AIVs) such as African black nightshade (Solanum nigrum) and African spiderplant (Cleome gynandra) involves either boiling and discarding the first water or lengthy boiling. Fermentation is considered a better alternative processing technique due to the enhanced retention of phytochemical contents and sensory properties. However, little is known about the impact of lactic acid fermentation on the phytochemical content, antioxidant capacity, sensory acceptability and microbial safety of the African black nightshade and African spiderplant. This study aimed to ferment AIVs using combined starter cultures (Lactobacillus fermentum and Lactococcus lactis) and further determine their effect on the phytochemical content (phenolic compounds and flavonoids), antioxidant capacity, sensory acceptability and microbial safety of the vegetables. There was a marked increase in phenol and flavonoid contents in all fermented vegetables (p < 0.05). The highest phenol content was 228.8 mg/g GAE (gallic acid equivalent) in the starter-culture-inoculated African black nightshade, while flavonoid content was 10.6 mg/g QE (quercetin equivalent) in the same. Starter-culture-inoculated AIVs presented significantly higher antioxidant capacity with a 60–80% radical scavenging activity compared to levels in uninoculated batches (p < 0.05). Fermented vegetables were more liked than the boiled vegetables and were microbiologically safe. In conclusion, lactic fermentation of AIVs increased phytochemical contents (phenolic compounds and flavonoids), maintained antioxidant capacity and improved product safety and sensory acceptability. Therefore, fermentation and consumption of the African indigenous vegetables are to be encouraged. Full article

Salmonella enterica is the principal causative agent of salmonellosis, a threat to human health. Because of its high antimicrobial resistance potential, Salmonella enterica has become worrisome, mostly in developing countries where hygiene and antimicrobial usage are defective. This study aimed to determine the epidemiology of the intestinal carriage of Extended Spectrum β-Lactamase producing Salmonella enterica from chickens and poultry farmers in Dschang, a town in the western region of Cameroon. A total of 416 chickens and 72 farmers were sampled between May and October 2020; and Salmonella enterica were isolated and subjected to extended spectrum β-lactamase screening. Logistic regression was used to test for statistical associations using a p-value of ≤0.05. Results from this study revealed that the prevalence of the intestinal carriage of Salmonella enterica for chickens and farmers were 55.77% [51.00; 60.54] and 22.22% [12.62; 31.82], respectively. Meanwhile, the intestinal carriage of Extended Spectrum β-Lactamase producing Salmonella enterica was 23.08% [13.76; 32.40] and 5.55% [0.26; 10.84] from chickens and poultry farmers, respectively. The risk factor for this carriage was revealed to be lack of knowledge by actors in livestock industries of antibiotic resistance. Chickens, just like poultry farmers, represent the starting point of community salmonellosis, which is difficult to cure; therefore, sensitization of breeders is an effective tool for the mitigation of this burden. Full article

Bacterial proteases participate in the proteolytic elimination of misfolded or aggregated proteins, carried out by members of the AAA+ protein superfamily such as Hsp100/Clp, Lon, and FtsH. It is estimated that the Clp and Lon families perform around 80% of cellular proteolysis in bacteria. These functions are regulated, in part, through the spatial and/or temporal use of adapter proteins, which participate in the recognition and delivery of specific substrate proteins to proteases. The proteolysis plays an important role in maintaining and controlling the quality of the proteins, avoiding the accumulation and aggregation of unfolded or truncated proteins. However, this is not their only function, since they play an important role in the formation of virulent phenotypes and in the response to different types of stress faced when entering the host or that occur in the environment. This review summarizes the structural and functional aspects of the Clp proteases and their role in Gram-positive microorganisms. Full article

African indigenous leafy vegetables (AILVs) are plants that have been part of the food systems in Sub-Saharan Africa (SSA) for a long time and their leaves, young shoots, flowers, fruits and seeds, stems, tubers, and roots are consumed. These vegetables are high in vitamins, minerals, protein, and secondary metabolites that promote health. This study aimed at isolating, characterizing, and identifying dominant lactic acid bacteria (LAB) from naturally fermenting commonly consumed AILV in Kenya. A total of 57 LAB strains were isolated and identified based on phenotypic and 16S rRNA gene analyses from three AILVs (23 nightshade leaves, 19 cowpeas leaves, and 15 vegetable amaranth). The highest microbial counts were recorded between 48 h and 96 h of fermentation in all AILVs ranging from approximately log 8 to log 9 CFU/mL with an average pH of 3.7. Fermentation of AILVs was dominated by twenty eight Lactobacillus spp. [Lactiplantibacillus plantarum (22), Limosilactobacillus fermentum (3), Lactiplantibacillus pentosus (2) and Lactiplantibacillus casei (1)], eleven Weissella spp. (Weissella cibaria (8), W. confusa (2), and W. muntiaci) six Leuconostoc spp. [Leuconostoc mesenteroides (3), Leuc. citreum (2) and Leuc. lactis (1)], six Pediococcus pentosaceus, four Enterococcus spp. [Enterococcus mundtii (2), E. faecalis (1) and E. durans (1)] and, finally, two Lactococcus garvieae. These bacteria strains are commonly used in food fermentation as starter cultures and as potential probiotics. Full article

Septicaemia is public health problem worldwide with a high rate of mortality among children. Epidemiological data on this phenomenon in Cameroon are still scarce. This study aimed to determine the prevalence and associated factors to septicaemia due to E. coli strains producing extended spectrum beta-lactamase (ESBL) in two hospitals in Yaoundé, Cameroon. A prospective, cross-sectional study was conducted on infants aged 0 to 2 years old at the consultation and neonatology care unit of two district hospitals of Yaoundé (UTHY and YGOPH) during a period of seven months (from August 2019 to March 2020). Each blood sample collected per infant was cultured in hemoline performance vials, and bacterial strains were identified using the Api-20 E system. In addition, an antibiotic resistant profile of isolates as well as the ESBL production were performed in accordance with the recommendations of the Antibiogram committee of the French Society of Microbiology 2019. Data were analysed in Epi-Info7.0 and for p less than 0.05, the difference was statistically significant. Of the 300 children enrolled, 130 (43.33%) were blood culture positive, and E. coli. was the most prevalent (69.23% (90/130)). Then antibiotic susceptibility test revealed that 77 over 90 E. coli strains were resistant to penicillin (with 85.55% to amoxicillin), and 34.44% were producing ESBL. Factors such as immunodeficiency, being on antibiotics, and particularly taking β-lactam were significantly associated with E. coli ESBL production ([aOR = 19.93; p = 0.0001], [aOR = 1.97; p = 0.04], and [aOR = 3.54; p = 0.01], respectively). Moreover, co-resistance to aminoglycosides, quinolones, fluoroquinolones, and cotrimoxazole were also found. This study highlighted a high prevalence of E. coli ESBL in blood samples of children aged 0–2 years in Yaoundé and prompts the development of more efficient strategies against E. coli ESBL associated mortality in infants in Cameroon. Full article